Spatial transcriptomics has emerged as a pivotal technique in understanding the complex microenvironment of gliomas, particularly in elucidating tumor heterogeneity and its implications for treatment. One notable study utilized diffusion tensor tractography (DTI) to perform along-tract analysis of white matter (WM) tracts in patients with diffuse gliomas. This study reconstructed five bilateral sets of WM tracts based on the HCP-1065 template, allowing for a detailed assessment of microstructural integrity. The researchers sampled diffusion-based parameters including fractional anisotropy (FA), radial diffusivity (RD), axial diffusivity (AD), mean diffusivity (MD), and quantitative anisotropy (QA) across 100 indices for each tract. The findings indicated significant alterations in these parameters, suggesting that gliomas not only disrupt local tissue architecture but also affect distant WM tracts, which could have implications for surgical planning and patient prognosis (ref: Fahlström doi.org/10.3390/brainsci14030213/). Furthermore, the study highlighted the potential of DTI as a non-invasive biomarker for assessing glioma infiltration and its impact on surrounding neural structures, emphasizing the need for integrating imaging modalities with spatial transcriptomics to gain a comprehensive understanding of glioma biology. This integrative approach could pave the way for personalized therapeutic strategies that target both tumor cells and the surrounding microenvironment.